Journal: Scientific Data

Article Title: The Spheroid Light Microscopy Image Atlas for morphometrical analysis of three-dimensional cell cultures

doi: 10.1038/s41597-025-04441-x

Figure Lengend Snippet: 2D cell culture conditions.

Article Snippet: HSC4 , JCRB/HSRRB (Japan) , DMEM LG , 1 , 1 , 25 , — , FBS , 10 , Yes , 5 , Yes.

Techniques: Cell Culture, Concentration Assay, Transfection

Journal: Frontiers in Oncology

Article Title: Inhibition of ATM or ATR in combination with hypo-fractionated radiotherapy leads to a different immunophenotype on transcript and protein level in HNSCC

doi: 10.3389/fonc.2024.1460150

Figure Lengend Snippet: Treatment scheme of HNSCC cell lines. HPV-negative (HSC4, Cal-33) and HPV-positive (UM-SCC-47, UD-SCC-2) HNSCC cells were seeded on day 1 and treated with 1µM AZD0156 or 0,1 µM VE-822 24 hours later. Irradiation with 5 Gy was performed 3 hours after treatment with smKI and repeated 24 hours later. Cells were harvested and analyzed 24 hours after the last treatment.

Article Snippet: Two HPV-negative HSC4 (RRID: CVCL_1289), Cal-33 (RRID: CVCL_1108) and two HPV-positive UM-SCC-47 (RRID: CVCL_7759), UD-SCC-2 (RRID: CVCL_E325) HNSCC cell lines were generously provided by Dr. Thorsten Rieckmann at the University Medical Center Hamburg-Eppendorf.

Techniques: Irradiation

Journal: Frontiers in Oncology

Article Title: Inhibition of ATM or ATR in combination with hypo-fractionated radiotherapy leads to a different immunophenotype on transcript and protein level in HNSCC

doi: 10.3389/fonc.2024.1460150

Figure Lengend Snippet: Cell death analysis of HNSCC treated with ATMi or ATRi w/o 2x5Gy RT. RT combined with ATRi shows the highest toxicity in HNSCC cells, regardless of the HPV status. 48 hours post-RT, HPV-negative HSC4 (A) and Cal-33 (B) cells as well as HPV-positive UM-SCC-47 (C) and UD-SCC-2 (D) cells were identified as viable (AnnexinV-, PI-), apoptotic (AnnexinV+, PI-) or necrotic (AnnexinV+, PI+). Percentages of viable, apoptotic, and necrotic cells are shown as stacked bars representing the mean ± SD. A one-tailed Mann-Whitney U test was performed to compare the different treatment approaches within one cell line: *p ≤ 0.05, n=4.

Article Snippet: Two HPV-negative HSC4 (RRID: CVCL_1289), Cal-33 (RRID: CVCL_1108) and two HPV-positive UM-SCC-47 (RRID: CVCL_7759), UD-SCC-2 (RRID: CVCL_E325) HNSCC cell lines were generously provided by Dr. Thorsten Rieckmann at the University Medical Center Hamburg-Eppendorf.

Techniques: One-tailed Test, MANN-WHITNEY

Journal: Frontiers in Oncology

Article Title: Inhibition of ATM or ATR in combination with hypo-fractionated radiotherapy leads to a different immunophenotype on transcript and protein level in HNSCC

doi: 10.3389/fonc.2024.1460150

Figure Lengend Snippet: Flow cytometric analysis of immune checkpoint surface marker on HNSCC after ATMi or ATRi w/o 2x5Gy RT. Combining RT with ATRi leads to the upregulation of immunostimulatory ICOS-L and CD137-L on HNSCC cells, irrespective of the HPV status. 48 hours after the last treatment, the HNSCC cells were harvested and the expression of immunostimulatory ICMs ICOS-L and CD137-L was examined by flow cytometry. The gating strategy is presented in (A) ICM expression is presented as ΔMFI (delta of mean fluorescence intensity of stained samples – background fluorescence) (B, C) . The combined treatment of RT and ATRi increased ICOS-L expression in all four cell lines compared to ATRi or RT alone, as well as in comparison to RT combined with ATMi (B) . Dual treatment with RT and ATMi tended to even decrease ICOS-L surface expression compared to RT or RT with ATRi (B) . RT and ATRi led to enhanced expression of immunostimulatory CD137-L in HPV-negative HSC4 cells compared to ATRi or RT alone, or the combined treatment of RT and ATMi (C) . In HPV-positive UM-SCC-47 and UD-SCC-2 cells, the combination of RT and ATRi also demonstrated higher surface expression of CD137-L compared to RT alone or treatment with RT and ATMi, respectively (C) . A one-tailed Mann-Whitney U test was performed to compare the different treatment approaches within one cell line: *p ≤ 0.05, n=4.

Article Snippet: Two HPV-negative HSC4 (RRID: CVCL_1289), Cal-33 (RRID: CVCL_1108) and two HPV-positive UM-SCC-47 (RRID: CVCL_7759), UD-SCC-2 (RRID: CVCL_E325) HNSCC cell lines were generously provided by Dr. Thorsten Rieckmann at the University Medical Center Hamburg-Eppendorf.

Techniques: Marker, Expressing, Flow Cytometry, Fluorescence, Staining, Comparison, One-tailed Test, MANN-WHITNEY